Articles | Volume 26, issue 22
Technical note
17 Nov 2022
Technical note |  | 17 Nov 2022

Technical note: On uncertainties in plant water isotopic composition following extraction by cryogenic vacuum distillation

Haoyu Diao, Philipp Schuler, Gregory R. Goldsmith, Rolf T. W. Siegwolf, Matthias Saurer, and Marco M. Lehmann

Related authors

The seasonal origins and ages of water provisioning streams and trees in a tropical montane cloud forest
Emily I. Burt, Gregory R. Goldsmith, Roxanne M. Cruz-de Hoyos, Adan Julian Ccahuana Quispe, and A. Joshua West
Hydrol. Earth Syst. Sci., 27, 4173–4186,,, 2023
Short summary
Siberian tree-ring and stable isotope proxies as indicators of temperature and moisture changes after major stratospheric volcanic eruptions
Olga V. Churakova (Sidorova), Marina V. Fonti, Matthias Saurer, Sébastien Guillet, Christophe Corona, Patrick Fonti, Vladimir S. Myglan, Alexander V. Kirdyanov, Oksana V. Naumova, Dmitriy V. Ovchinnikov, Alexander V. Shashkin, Irina P. Panyushkina, Ulf Büntgen, Malcolm K. Hughes, Eugene A. Vaganov, Rolf T. W. Siegwolf, and Markus Stoffel
Clim. Past, 15, 685–700,,, 2019
Short summary
Seasonal origins of soil water used by trees
Scott T. Allen, James W. Kirchner, Sabine Braun, Rolf T. W. Siegwolf, and Gregory R. Goldsmith
Hydrol. Earth Syst. Sci., 23, 1199–1210,,, 2019
Short summary
Ideas and perspectives: Tracing terrestrial ecosystem water fluxes using hydrogen and oxygen stable isotopes – challenges and opportunities from an interdisciplinary perspective
Daniele Penna, Luisa Hopp, Francesca Scandellari, Scott T. Allen, Paolo Benettin, Matthias Beyer, Josie Geris, Julian Klaus, John D. Marshall, Luitgard Schwendenmann, Till H. M. Volkmann, Jana von Freyberg, Anam Amin, Natalie Ceperley, Michael Engel, Jay Frentress, Yamuna Giambastiani, Jeff J. McDonnell, Giulia Zuecco, Pilar Llorens, Rolf T. W. Siegwolf, Todd E. Dawson, and James W. Kirchner
Biogeosciences, 15, 6399–6415,,, 2018
Short summary
Inter-laboratory comparison of cryogenic water extraction systems for stable isotope analysis of soil water
Natalie Orlowski, Lutz Breuer, Nicolas Angeli, Pascal Boeckx, Christophe Brumbt, Craig S. Cook, Maren Dubbert, Jens Dyckmans, Barbora Gallagher, Benjamin Gralher, Barbara Herbstritt, Pedro Hervé-Fernández, Christophe Hissler, Paul Koeniger, Arnaud Legout, Chandelle Joan Macdonald, Carlos Oyarzún, Regine Redelstein, Christof Seidler, Rolf Siegwolf, Christine Stumpp, Simon Thomsen, Markus Weiler, Christiane Werner, and Jeffrey J. McDonnell
Hydrol. Earth Syst. Sci., 22, 3619–3637,,, 2018
Short summary

Related subject area

Subject: Ecohydrology | Techniques and Approaches: Uncertainty analysis
Technical note: A procedure to clean, decompose, and aggregate time series
François Ritter
Hydrol. Earth Syst. Sci., 27, 349–361,,, 2023
Short summary
Macroinvertebrate habitat requirements in rivers: overestimation of environmental flow calculations in incised rivers
Renata Kędzior, Małgorzata Kłonowska-Olejnik, Elżbieta Dumnicka, Agnieszka Woś, Maciej Wyrębek, Leszek Książek, Jerzy Grela, Paweł Madej, and Tomasz Skalski
Hydrol. Earth Syst. Sci., 26, 4109–4124,,, 2022
Short summary
Impacts of non-ideality and the thermodynamic pressure work term pΔv on the surface energy balance
William J. Massman
Hydrol. Earth Syst. Sci., 24, 967–975,,, 2020
Short summary
How to predict hydrological effects of local land use change: how the vegetation parameterisation for short rotation coppices influences model results
F. Richter, C. Döring, M. Jansen, O. Panferov, U. Spank, and C. Bernhofer
Hydrol. Earth Syst. Sci., 19, 3457–3474,,, 2015
Short summary
Modelling irrigated maize with a combination of coupled-model simulation and uncertainty analysis, in the northwest of China
Y. Li, W. Kinzelbach, J. Zhou, G. D. Cheng, and X. Li
Hydrol. Earth Syst. Sci., 16, 1465–1480,,, 2012

Cited articles

Allen, S. T. and Kirchner, J. W.: Potential effects of cryogenic extraction biases on plant water source partitioning inferred from xylem water isotope ratios, Hydrol. Process., 36, e14483,, 2022. 
Allen, S. T., Kirchner, J. W., Braun, S., Siegwolf, R. T. W., and Goldsmith, G. R.: Seasonal origins of soil water used by trees, Hydrol. Earth Syst. Sci., 23, 1199–1210,, 2019. 
Barbeta, A., Gimeno, T. E., Clavé, L., Fréjaville, B., Jones, S. P., Delvigne, C., Wingate, L., and Ogée, J.: An explanation for the isotopic offset between soil and stem water in a temperate tree species, New Phytol., 227, 766–779,, 2020. 
Barbeta, A., Burlett, R., Martín-Gómez, P., Fréjaville, B., Devert, N., Wingate, L., Domec, J.-C., and Ogée, J.: Evidence for distinct isotopic compositions of sap and tissue water in tree stems: consequences for plant water source identification, New Phytol., 233, 1121–1132,, 2022. 
Bowers, W. H. and Williams, D. G.: Isotopic Heterogeneity of Stem Water in Conifers Is Correlated to Xylem Hydraulic Traits and Supports Multiple Residence Times, Frontiers in Water, 4, 861590,, 2022. 
Short summary
We systematically investigate the uncertainties in previously observed isotopic offsets between plant source water and water extracted by cryogenic vacuum distillation. Our results show that hydrogen isotope exchange between organic material and water is a real phenomenon. However, the isotopic offsets are rather influenced by the actual amount of extracted water, sublimation, and evaporation. Our findings will help improve interpretations of ecohydrological processes in isotope-based studies.