Articles | Volume 26, issue 13
https://doi.org/10.5194/hess-26-3673-2022
https://doi.org/10.5194/hess-26-3673-2022
Research article
 | 
14 Jul 2022
Research article |  | 14 Jul 2022

A comparative study of plant water extraction methods for isotopic analyses: Scholander-type pressure chamber vs. cryogenic vacuum distillation

Giulia Zuecco, Anam Amin, Jay Frentress, Michael Engel, Chiara Marchina, Tommaso Anfodillo, Marco Borga, Vinicio Carraro, Francesca Scandellari, Massimo Tagliavini, Damiano Zanotelli, Francesco Comiti, and Daniele Penna

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Cited articles

Aguzzoni, A., Engel, M., Zanotelli, D., Penna, D., Comiti, F., and Tagliavini, M.: Water uptake dynamics in apple trees assessed by an isotope labeling approach, Agr. Water Manage., 266, 107572, https://doi.org/10.1016/j.agwat.2022.107572, 2022. a
Allen, S. T. and Kirchner, J. W.: Potential effects of cryogenic extraction biases on plant water source partitioning inferred from xylem-water isotope ratios, Hydrol. Process., 36, e14483, https://doi.org/10.1002/hyp.14483, 2022. a, b, c, d
Allen, S. T., Kirchner, J. W., Braun, S., Siegwolf, R. T. W., and Goldsmith, G. R.: Seasonal origins of soil water used by trees, Hydrol. Earth Syst. Sci., 23, 1199–1210, https://doi.org/10.5194/hess-23-1199-2019, 2019. a
Amin, A., Zuecco, G., Geris, J., Schwendenmann, L., McDonnell, J. J., Borga, M., and Penna, D.: Depth distribution of soil water sourced by plants at the global scale: a new direct inference approach, Ecohydrology, 13, e2177, https://doi.org/10.1002/eco.2177, 2020. a
Amin, A., Zuecco, G., Marchina, C., Engel, M., Penna, D., McDonnell, J. J., and Borga M.: No evidence of isotopic fractionation in olive trees (Olea europaea): a stable isotope tracing experiment, Hydrolog. Sci. J., 66, 2415–2430, https://doi.org/10.1080/02626667.2021.1987440, 2021. a
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Short summary
We analyzed the variability in the isotopic composition of plant water extracted by two different methods, i.e., cryogenic vacuum distillation (CVD) and Scholander-type pressure chamber (SPC). Our results indicated that the isotopic composition of plant water extracted by CVD and SPC was significantly different. We concluded that plant water extraction by SPC is not an alternative for CVD as SPC mostly extracts the mobile plant water whereas CVD retrieves all water stored in the sampled tissue.