Articles | Volume 26, issue 13
https://doi.org/10.5194/hess-26-3673-2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/hess-26-3673-2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
14 Jul 2022
Research article |
| 14 Jul 2022
| 14 Jul 2022
A comparative study of plant water extraction methods for isotopic analyses: Scholander-type pressure chamber vs. cryogenic vacuum distillation
Department of Land, Environment, Agriculture and Forestry, University of Padova, Legnaro (PD), Italy
Anam Amin
Department of Land, Environment, Agriculture and Forestry, University of Padova, Legnaro (PD), Italy
Jay Frentress
Faculty of Science and Technology, Free University of Bozen-Bolzano, Bozen-Bolzano, Italy
Michael Engel
Faculty of Science and Technology, Free University of Bozen-Bolzano, Bozen-Bolzano, Italy
Chiara Marchina
Department of Land, Environment, Agriculture and Forestry, University of Padova, Legnaro (PD), Italy
Tommaso Anfodillo
Department of Land, Environment, Agriculture and Forestry, University of Padova, Legnaro (PD), Italy
Marco Borga
Department of Land, Environment, Agriculture and Forestry, University of Padova, Legnaro (PD), Italy
Vinicio Carraro
Department of Land, Environment, Agriculture and Forestry, University of Padova, Legnaro (PD), Italy
Francesca Scandellari
Isotracer s.r.l., Bologna, Italy
Massimo Tagliavini
Faculty of Science and Technology, Free University of Bozen-Bolzano, Bozen-Bolzano, Italy
Damiano Zanotelli
Faculty of Science and Technology, Free University of Bozen-Bolzano, Bozen-Bolzano, Italy
Francesco Comiti
Faculty of Science and Technology, Free University of Bozen-Bolzano, Bozen-Bolzano, Italy
Daniele Penna
Department of Agriculture, Food, Environment and Forestry, University of Florence, Florence, Italy
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Short summary
We analyzed the variability in the isotopic composition of plant water extracted by two different methods, i.e., cryogenic vacuum distillation (CVD) and Scholander-type pressure chamber (SPC). Our results indicated that the isotopic composition of plant water extracted by CVD and SPC was significantly different. We concluded that plant water extraction by SPC is not an alternative for CVD as SPC mostly extracts the mobile plant water whereas CVD retrieves all water stored in the sampled tissue.
We analyzed the variability in the isotopic composition of plant water extracted by two...
