Articles | Volume 25, issue 9
https://doi.org/10.5194/hess-25-5219-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/hess-25-5219-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Technical note
| 
27 Sep 2021
Technical note |
| 27 Sep 2021
| 27 Sep 2021
Technical note: Unresolved aspects of the direct vapor equilibration method for stable isotope analysis (δ18O, δ2H) of matrix-bound water: unifying protocols through empirical and mathematical scrutiny
Benjamin Gralher
CORRESPONDING AUTHOR
Chair of Hydrology, University of Freiburg, Freiburg 79098, Germany
Barbara Herbstritt
Chair of Hydrology, University of Freiburg, Freiburg 79098, Germany
Markus Weiler
Chair of Hydrology, University of Freiburg, Freiburg 79098, Germany
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16 citations as recorded by crossref.
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- Technical note: A fast and reproducible autosampler for direct vapor equilibration isotope measurements J. Pyschik et al. 10.5194/hess-29-525-2025
- Influence of equilibration time, soil texture, and saturation on the accuracy of porewater water isotope assays using the direct H2O(liquid)–H2O(vapor) equilibration method D. Vadibeler et al. 10.1016/j.jhydrol.2022.127560
- Influencing Factors and Calibration of the Direct Vapor Equilibration Method for Measuring Soil Water Isotopes Z. Xing et al. 10.3390/w17010116
- Characterizing the heterogeneity of eastern hemlock xylem water isotopic compositions: Implications for the design of plant water uptake studies K. Li & J. Knighton 10.1002/eco.2571
- Quantification of water fluxes and soil water balance in agricultural fields under different tillage and irrigation systems using water stable isotopes A. Canet-Martí et al. 10.1016/j.still.2023.105732
- On the urgent need for standardization in isotope‐based ecohydrological investigations C. Millar et al. 10.1002/hyp.14698
- Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems R. Magh et al. 10.5194/hess-26-3573-2022
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14 citations as recorded by crossref.
- Evidence for variations in cryogenic extraction deuterium biases of plant xylem water across foundational northeastern US trees M. Sobota et al. 10.1002/hyp.15079
- The influence of hillslope topography on beech water use: a comparative study in two different climates G. Fabiani et al. 10.5194/hess-28-2683-2024
- Toward a common methodological framework for the sampling, extraction, and isotopic analysis of water in the Critical Zone to study vegetation water use N. Ceperley et al. 10.1002/wat2.1727
- Influence of sample preparation procedures on water stable isotopes in plant organs using the water‐vapour equilibrium method S. Santos Pires et al. 10.1002/eco.2444
- Estimates of water partitioning in complex urban landscapes with isotope‐aided ecohydrological modelling M. Gillefalk et al. 10.1002/hyp.14532
- Oxygen isotope ratios of phosphates in the soil‐plant system: Limitations and future developments C. von Sperber et al. 10.1111/ejss.13434
- Technical note: Discrete in situ vapor sampling for subsequent lab-based water stable isotope analysis B. Herbstritt et al. 10.5194/hess-27-3701-2023
- Technical note: A fast and reproducible autosampler for direct vapor equilibration isotope measurements J. Pyschik et al. 10.5194/hess-29-525-2025
- Influence of equilibration time, soil texture, and saturation on the accuracy of porewater water isotope assays using the direct H2O(liquid)–H2O(vapor) equilibration method D. Vadibeler et al. 10.1016/j.jhydrol.2022.127560
- Influencing Factors and Calibration of the Direct Vapor Equilibration Method for Measuring Soil Water Isotopes Z. Xing et al. 10.3390/w17010116
- Characterizing the heterogeneity of eastern hemlock xylem water isotopic compositions: Implications for the design of plant water uptake studies K. Li & J. Knighton 10.1002/eco.2571
- Quantification of water fluxes and soil water balance in agricultural fields under different tillage and irrigation systems using water stable isotopes A. Canet-Martí et al. 10.1016/j.still.2023.105732
- On the urgent need for standardization in isotope‐based ecohydrological investigations C. Millar et al. 10.1002/hyp.14698
- Technical note: Conservative storage of water vapour – practical in situ sampling of stable isotopes in tree stems R. Magh et al. 10.5194/hess-26-3573-2022
2 citations as recorded by crossref.
- Seasonal variations in soil–plant interactions in contrasting urban green spaces: Insights from water stable isotopes C. Marx et al. 10.1016/j.jhydrol.2022.127998
- Flushing or mixing? Stable water isotopes reveal differences in arctic forest and peatland soil water seasonality F. Muhic et al. 10.1002/hyp.14811
Latest update: 02 Jul 2025
Short summary
We scrutinized the quickest currently available method for stable isotope analysis of matrix-bound water. Simulating common procedures, we demonstrated the limits of certain materials currently used and identified a reliable and cost-efficient alternative. Further, we calculated the optimum proportions of important protocol aspects critical for precise and accurate analyses. Our unifying protocol suggestions increase data quality and comparability as well as the method's general applicability.
We scrutinized the quickest currently available method for stable isotope analysis of...
