Research on groundwater-dependent ecosystems (GDEs) started earlier, but because there is no good identification and classification method, most of the related research is concentrated in Europe and Australia. In this study, the lower Yellow River basin in northern China, with well-developed karsts, was selected as the study area, and a four-diagnostic-criteria framework for identifying the GDEs based on remote sensing, GIS data dredging, and hydrogeological surveys was proposed.